bam hi restriction enzyme Search Results


bam hi enzyme  (Promega)
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Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Bam Hi Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bam hi restriction enzyme  (Promega)
90
Promega bam hi restriction enzyme
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Bam Hi Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes sal i and bam hi  (Promega)
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Promega restriction enzymes sal i and bam hi
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Restriction Enzymes Sal I And Bam Hi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes hin d iii, bam h i  (Promega)
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Promega restriction enzymes hin d iii, bam h i
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Restriction Enzymes Hin D Iii, Bam H I, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bam hi enzyme  (GenStar Biosolutions)
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GenStar Biosolutions bam hi enzyme
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Bam Hi Enzyme, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bam hi and not i restriction enzymes  (Promega)
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Promega bam hi and not i restriction enzymes
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Bam Hi And Not I Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kpn i enzyme  (Promega)
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Promega kpn i enzyme
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Kpn I Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bam hi/ sal i restriction enzymes  (Promega)
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Promega bam hi/ sal i restriction enzymes
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Bam Hi/ Sal I Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes eco rv and bam hi  (NZYTech Inc)
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NZYTech Inc restriction enzymes eco rv and bam hi
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Restriction Enzymes Eco Rv And Bam Hi, supplied by NZYTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes eco ri and bam hi  (Shanghai GenePharma)
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Shanghai GenePharma restriction enzymes eco ri and bam hi
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Restriction Enzymes Eco Ri And Bam Hi, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes pvu ii, pst i, bam hi, sac i, and bgl ii  (Promega)
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Promega restriction enzymes pvu ii, pst i, bam hi, sac i, and bgl ii
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Restriction Enzymes Pvu Ii, Pst I, Bam Hi, Sac I, And Bgl Ii, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bam hi and nde i restriction enzymes  (GenScript corporation)
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GenScript corporation bam hi and nde i restriction enzymes
Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products
Bam Hi And Nde I Restriction Enzymes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products

Journal: Memórias do Instituto Oswaldo Cruz

Article Title: Stable expression of Mycobacterium bovis antigen 85B in auxotrophic M. bovis bacillus Calmette-Guérin

doi: 10.1590/0074-02760160360

Figure Lengend Snippet: Strains, primers, and restriction enzymes used for cloning of various portions of Mycobacterium bovis fbpB and its protein products

Article Snippet: The PCR product was digested with Bam HI and Hind III enzymes (Promega) and inserted into the pAE vector , which had been previously digested with the same restriction enzymes.

Techniques: Cloning, Expressing, Recombinant

: schematic outline of the process of constructing recombinant plasmids for Ag85B expression in Mycobacterium bovis bacillus Calmette-Guérin. The DNA fragments cloned into vector pUP410 were previously amplified by polymerase chain reaction (PCR) from M. bovis genomic DNA. (A) Cloning of the fbp B coding region and its endogenous promoter, resulting in pUP410::85B; (B) cloning of a portion of fbpB that encodes the mature protein. First, a portion of the fbpB coding region (120 to 978 bp) was cloned into vector pUS2000, yielding pUS2000::85BT. From the resulting recombinant vector, an 1149-bp amplicon containing the cloned fragment under control of an 18-kDa promoter from M. leprae was obtained by PCR and then cloned into pUP410, yielding pUP410::85BT. The kanamycin resistance gene was removed by digestion with the Hind III enzyme, and the digestion product was ligated using the T4 ligase enzyme.

Journal: Memórias do Instituto Oswaldo Cruz

Article Title: Stable expression of Mycobacterium bovis antigen 85B in auxotrophic M. bovis bacillus Calmette-Guérin

doi: 10.1590/0074-02760160360

Figure Lengend Snippet: : schematic outline of the process of constructing recombinant plasmids for Ag85B expression in Mycobacterium bovis bacillus Calmette-Guérin. The DNA fragments cloned into vector pUP410 were previously amplified by polymerase chain reaction (PCR) from M. bovis genomic DNA. (A) Cloning of the fbp B coding region and its endogenous promoter, resulting in pUP410::85B; (B) cloning of a portion of fbpB that encodes the mature protein. First, a portion of the fbpB coding region (120 to 978 bp) was cloned into vector pUS2000, yielding pUS2000::85BT. From the resulting recombinant vector, an 1149-bp amplicon containing the cloned fragment under control of an 18-kDa promoter from M. leprae was obtained by PCR and then cloned into pUP410, yielding pUP410::85BT. The kanamycin resistance gene was removed by digestion with the Hind III enzyme, and the digestion product was ligated using the T4 ligase enzyme.

Article Snippet: The PCR product was digested with Bam HI and Hind III enzymes (Promega) and inserted into the pAE vector , which had been previously digested with the same restriction enzymes.

Techniques: Recombinant, Expressing, Clone Assay, Plasmid Preparation, Amplification, Polymerase Chain Reaction, Cloning, Control